Sample Lectures
Introduction to BioSeparations
Synthetic molecules are produced with a few purified reactants and produce a major product with a few by-products. The desired compound can typically be purified with one or two separation steps. Biologically-produced molecules are often in low concentration awash in orders-of-magnitude more cellular products. Many of these molecules are nearly identical in physiochemical properties to the target compound of interest. This means bioseparations are complex requiring serial combination of multiple different separation steps to get to the desired product.
Ionization
Most biomolecules are ions within the physiological pH range. In this lecture we discuss the mathematics of ionization and and how this physiochemical property can be exploited for the separation of similar compounds by solubility, crystalization, ion exchange, and electrophoresis. We demonstrate spreadsheet tools (available as a book resourses on this website) for predicting protein properties, like isoelectric point, and to calculate the pH of complex ionic mixtures.
Liquid Chromatography
In this video we describe the mathematical models for both capillary and packed column liquid chromatography. When these models are made dimensionless, we generate 4 dimensionless numbers (Peclet, Biot, gamma, and xi). By holding these dimensionless numbers constant we can easily scale-up any liquid chromatography process. A spreadsheet complete with pressure and L/D constraints is demonstrated for this purpose.. This spreadsheet can be obtained as part of the textbook web resources on this site.
Electrophoresis
In this lecture we describe the mathematical models to describe electrophoretic separations. We present both rigorous and more practical models for the electrophoretic process.
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